Michael McIntosh

Michael McIntosh,

Associate Professor

Department: MD-CHILD HEALTH RES INSTITUTE
Business Phone: (352) 294-8870
Business Email: mmcintosh@peds.ufl.edu

Research Profile

My scientific career has spanned basic and applied research in human and veterinary medicine in academia and government. Dedicated to the control and prevention of infectious diseases through research and education, my research combines advanced molecular biology and bioinformatics with traditional microbiology to reveal connections between infectious agents and diseases of unknown etiology or emerging epidemiology. Emphasis is on development of computational tools for analysis of conserved structural features of RNA and applications of transcriptomics and non-biased molecular sequencing to detect and characterize potential pathogens, investigate host-microbial interactions, and reveal underlying mechanisms of disease as well as targets for diagnosis or intervention. With the USDA, my laboratory’s development of advanced molecular methods for virus detection, including panviral microarrays and next-generation sequencing led to impactful examples of disease detection and response on national and international scales, most notably, the discovery of domestic swine as a host for Ebola. Now at the University of Florida, my research employs RNA-seq and capture-seq platforms to study transcriptional networks associated with gammaherpesvirus infections, and to discover new viruses in human diseases of unknown etiology or emerging epidemiology, at the human-animal or vector interface, and in the context of immunocompromise or cancer. In this regard, I combine virus discovery and transcriptomics to reveal new diagnostic markers, host factors contributing to disease, and targets for neutralizing antibody epitopes in virus surface proteins for the development of vaccines and antibody therapeutics.

Publications

2021
A heterochromatin inducing protein differentially recognizes self versus foreign genomes.
PLoS pathogens. 17(3) [DOI] 10.1371/journal.ppat.1009447. [PMID] 33730092.
2021
An Ancestral Retrovirus Envelope Protein Regulates Persistent Gammaherpesvirus Lifecycles.
Frontiers in microbiology. 12 [DOI] 10.3389/fmicb.2021.708404. [PMID] 34434177.
2021
Inflammasome, the Constitutive Heterochromatin Machinery, and Replication of an Oncogenic Herpesvirus.
Viruses. 13(5) [DOI] 10.3390/v13050846. [PMID] 34066537.
2020
A Mechanism-Based Targeted Screen To Identify Epstein-Barr Virus-Directed Antiviral Agents.
Journal of virology. 94(21) [DOI] 10.1128/JVI.01179-20. [PMID] 32796077.
2020
Nascent Transcriptomics Reveal Cellular Prolytic Factors Upregulated Upstream of the Latent-to-Lytic Switch Protein of Epstein-Barr Virus.
Journal of virology. 94(7) [DOI] 10.1128/JVI.01966-19. [PMID] 31941784.
2020
STAT3 imparts BRCAness by impairing homologous recombination repair in Epstein-Barr virus-transformed B lymphocytes.
PLoS pathogens. 16(10) [DOI] 10.1371/journal.ppat.1008849. [PMID] 33002095.
2019
Novel replisome-associated proteins at cellular replication forks in EBV-transformed B lymphocytes.
PLoS pathogens. 15(12) [DOI] 10.1371/journal.ppat.1008228. [PMID] 31841561.
2017
Development of a Feature and Template-Assisted Assembler and Application to the Analysis of a Foot-and-Mouth Disease Virus Genotyping Microarray.
PloS one. 12(1) [DOI] 10.1371/journal.pone.0166870. [PMID] 28045937.
2016
Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA.
Virology journal. 13 [DOI] 10.1186/s12985-016-0552-0. [PMID] 27260412.
2015
Detection of African swine fever, classical swine fever, and foot-and-mouth disease viruses in swine oral fluids by multiplex reverse transcription real-time polymerase chain reaction.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 27(2):140-9 [DOI] 10.1177/1040638715574768. [PMID] 25776540.
2013
Development of a universal RT-PCR for amplifying and sequencing the leader and capsid-coding region of foot-and-mouth disease virus.
Journal of virological methods. 189(1):70-6 [DOI] 10.1016/j.jviromet.2013.01.009. [PMID] 23380590.
2013
In situ surface-enhanced Raman spectroscopic study of formic acid electrooxidation on spontaneously deposited platinum on gold.
Physical chemistry chemical physics : PCCP. 15(24):9716-25 [DOI] 10.1039/c3cp51128f. [PMID] 23674096.
2012
Diagnosis of Porcine teschovirus encephalomyelitis in the Republic of Haiti.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 24(4):671-8 [DOI] 10.1177/1040638712445769. [PMID] 22604774.
2011
Comparison of methods for improved RNA extraction from blood for early detection of Classical swine fever virus by real-time reverse transcription polymerase chain reaction.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 23(4):727-35 [DOI] 10.1177/1040638711407685. [PMID] 21908315.
2011
Current perspectives on the phylogeny of Filoviridae.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 11(7):1514-9 [DOI] 10.1016/j.meegid.2011.06.017. [PMID] 21742058.
2011
Development, optimization, and validation of a Classical swine fever virus real-time reverse transcription polymerase chain reaction assay.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 23(5):994-8 [DOI] 10.1177/1040638711416970. [PMID] 21908362.
2009
Discovery of swine as a host for the Reston ebolavirus.
Science (New York, N.Y.). 325(5937):204-6 [DOI] 10.1126/science.1172705. [PMID] 19590002.

Grants

Sep 2021 ACTIVE
Epstein-Barr virus and B-cell co-opted functions of the endogenous retrovirus envelope protein, Syncytin-1
Role: Other
Funding: NATL INST OF HLTH NIDCR
Sep 2019 ACTIVE
Recombinant Adeno associated virus as a long-acting passive antibody vaccine against foreign and transboundary animal diseases
Role: Principal Investigator
Funding: US DEPT OF HOMELAND SECURITY

Education

PhD, Molecular Biology and Biotechnology
1995 · Drexel Medical College, PA (formerly Medical College of Pennsylvania & Hahnemann University)
BS, Molecular and Cellular Biology
1987 · The Pennsylvania State University, State College, PA

Teaching Profile

Courses Taught
2018
GMS7979 Advanced Research

Contact Details

Phones:
Business:
(352) 294-8870
Emails: