Chen Ling, Ph.D.

Chen-LingDivision

Cellular and Molecular Therapy

Academic Title

Research Assistant Professor

Contact Information

352-273-6174 (phone)
lingchen@peds.ufl.edu

Office

Cancer & Genetics Research Complex, Room 497

Lab

Cancer & Genetics Research Complex, Rooms 485G

Research Overview

Dr. Ling’s research focuses on adeno-associated virus (AAV) virology, gene therapy and traditional Chinese medicine (TCM). His laboratory has made contributions to the above fields including: identification of a novel function of the inverted terminal repeat in the AAV genome and a new method applied by AAV to reduce its endogenous gene expression; elucidation of cellular receptor/co-receptor for AAV serotype 3; development of human liver targeting recombinant AAV gene therapy vector; identification of small molecules isolated from TCM to enhance gene therapy outcome; and delivery of cytotoxic genes isolated from TCM for the purpose of cancer gene therapy. The current emphasis of Dr. Ling’s research is on combining the treatment of rAAV-based cancer gene therapy and of TCM for human hepatocellular carcinoma.

Training

  • B.S in Biological Science, Fudan University, China, 2007
  • Ph.D. in Biomedical Science – Genetics, University of Florida, USA, 2011
  • Postdoctoral Associate, Department of Pediatrics, University of Florida, 2011-2012

Key Publications

(# Corresponding author, * Co-first author)

Articles

  1. Ling C*#, Yin Z*, Li J, Zhang D, Aslanidi G, Srivastava A#. (2016) Strategies to generate high-titer, high-potency recombinant AAV3 serotype vectors. Mol Ther Methods Clin Dev. 4;3:16029.
  2. Sayroo R, Nolasco D, Yin Z, Cortes C. Y, Pandya M, Ling C# & Aslanidi G#. (2016) Development of novel AAV serotype 6 based vectors with selective tropism for human cancer cells. Gene Therapy. 23(1):18-25. PMID: 26270885.
  3. Li S*, Ling C*, Zhong L*, Li M, Su Q, He R, Tang Q, Greiner L. D, Shultz D. L, Brehm A. M, Flotte R. T, Mueller C,  Srivastava A#, & GaoG#. (2015) Efficient and targeted transduction of nonhuman primate liver with systemically delivered optimized AAV3B vectors. Molecular Therapy. 23(12):1867-76. PMID: 26403887; PMCID: PMC4700112.
  4. Ling C*, Wang Y*, Feng YL, Zhang YN, Li J, Hu XR, Wang LN, Zhong MF, Zhai XF, Zolotukhin I, Srivastava A# & Ling CQ#. (2015) Prevalence of neutralizing antibodies against liver-tropic adeno-associated virus serotype vectors in 100 healthy Chinese and its potential relation to body constitutions. Journal of Integrative Medicine, 13(5):341-6. PMID: 26343106.
  5. Pandya M, Britt K, Hoffman B, Ling C# & Aslanidi G#. (2015) Reprogramming immune response with capsid-optimized AAV6 vectors for immunotherapy of cancer. Journal of Immunotherapy, 38(7):292-8. PMID: 26261893; PMCID: PMC4535186.
  6. Wang L, Yin Z, Wang Y, Lu Y, Zhang D, Srivastava A, Ling CQ, Aslanidi GV# & Ling C#. (2015) Productive life cycle of the adeno-associated virus 2 in the complete absence of a conventional polyadenylation signal. Journal of General Virology, 96(9):2780-7. PMID: 26297494; PMCID: PMC4635500.
  7. Ling C#*, Wang Y*, Lu Y, Wang LN, Jayandharan G, Aslanidi V. G, Li B, Cheng B, Ma W, Lentz T, Ling CQ, Xiao X, Samulski R. J, Muzyczka N & Srivastava A#. (2015) Enhanced transgene expression from recombinant single-stranded AAV vectors in human cell lines in vitro and in murine hepatocytes in vivo. Journal of Virology, 89(2):952-61. PMID: 25355884; PMCID: PMC4300666.
  8. Ling C#*, Wang Y*, Zhang YH, Ejjigani A, Yin Zifei, Lu Y, Wang LN, Wang M, Li Jun, Hu Z, Aslanidi GV, Zhong Li, Gao G, Srivastava A# & Ling CQ#. (2014) Selective in vivo targeting of human liver tumors by optimized AAV3 vectors in a murine xenograft model. Human Gene Therapy, 25(12):1023-34. PMID: 25296041; PMCID: PMC4270149.
  9. Zhang YH, Wang Y, Yusufali A, Ashby F, Zhang D, Yin ZF, Aslanidi V. G, Srivastava A, Ling CQ# & Ling C#. (2014) Cytotoxic Genes from Traditional Chinese Medicine Inhibit Tumor Growth both in vitro and in vivo. Journal of Integrative Medicine, 12(6):483-94. PMID: 25412666.
  10. Wang L, Wang Y, Yin Z, Zhang Y, Aslanidi G, Srivastava A, Ling CQ# and Ling C#. (2014) Pristimerin enhances recombinant adeno-associated virus vector-mediated transgene expression in human cell lines in vitro and murine hepatocytes in vivo. Journal of Integrative Medicine, 12(1):20-34. PMID: 24461592.
  11. Cheng B*, Ling C*, Dai Y, Lu Y, Glushakova L.G, Gee S.W.Y., McGoogan K.E., Aslanidi G.V., Park M, Stacpoole P.W., Siemann D, Liu C, Srivastava A# & Ling CQ# (2012). Development of optimized AAV3 serotype vectors: Mechanism of high-efficiency transduction of human liver cancer cells. Gene Therapy, 19(4): 375-84. PMID: 21776025; PMCID: PMC3519243.
  12. Wang Y*, Ling C*, Song L, Wang L, Aslanidi G, Tan M, Ling CQ# & Srivastava A#. (2012). Limitations of encapsidation of recombinant scAAV2 genomes in different serotype capsids and their quantitation. Human Gene Therapy Methods, 23(4):225-33. PMID: 22966785; PMCID: PMC4015078.
  13. Ling C, Lu Y, Kelsi JK, Jayandharan GR, Li B, Ma W, Cheng B, Gee SW, McGoogan KE, Govindasamy L, Zhong L, Agbandje-McKenna M & Srivastava A#. (2010). Human Hepatocyte Growth Factor Receptor is a Cellular Co-Receptor for AAV3. Hum Gene Therapy, 21(12), 1741-1747. PMID: 20545554; PMCID: PMC2999576.

Reviews

  1. Feng Y, Yin Z, Zhang D, Srivastava A#, and Ling C#. (2015) Chinese medicine protein and peptide in gene and cell therapy. Current Protein and Peptide Science, in press
  2. Ling C*, Zhang Y*, Li J, Chen W#, Ling C# (2015) Clinical use of toxic proteins and peptides from Tian Hua Fen and scorpion venom. Current Protein and Peptide Science, in press
  3. Ling CQ#, Yue XQ & Ling C. (2014) Three advantages of using traditional Chinese medicine to prevent and treat tumor. Journal of Integrative Medicine, 12 (4), 331-335. PMID: 25074882.
  4. Ling CQ#*, Wang L*, Wang Y, Zhang Y, Yin Z, Wang M & Ling C#. (2014) The Roles of Traditional Chinese Medicine in Gene Therapy. Journal of Integrative Medicine, 12(2):67-75. PMID: 24666672.

Book Chapter

  1. Ling C & Zhang D, Adeno-associated virus and vector. In: Nanotechnology in Gene Delivery (edited by Drs. Yarong Liu, Pin Wang), Chapter 2, 48pp. The World Scientific Encyclopedia of Nanomedicine and Bioengineering II: Biosensing, Gene Delivery, and Tissue Regeneration, Volume 3 (Editor-in-chief: Dr. Donglu Shi).